Mol Cell Biochem. In the study of the oral glucose tolerance test result showed the better utilization of the glucose by the animals. DM occurs due to hereditary and environmental causes, resulting in abnormally high blood sugar level [1, 2]. Ahmadi SA, Boroumand MA, Gohari-Moghaddam K, Tajik P, Dibaj SM: The impact of Low serum triglyceride on LDL-cholesterol estimation. Acta Veterinaria Brno. The collected leaves of PF were gently washed with tap water to remove the extraporeneous matter. Google Scholar. The samples were obtained from the same liver anatomical regions. A voucher specimen (Ref. In diabetes, increased level of total cholesterol, is one of the major factors for coronary heart disease (hyperlipidaemia) and its incidence of atherosclerosis. belongs to the family Rubiaceae and is a climbing plant found in the Central and Eastern Himalayas, at elevations up to 5000 ft (approx. When STZ induced diabetic rat treated with different doses of PF, there was a significant (P < 0.001) decreased the level of glucose-6-phosphatase in dose dependent manner. leaves extract effect on pancreas histopathology in normal and STZ induced diabetic rats. prior to oral administration of ethanolic extract of P. foetida (200 mg/kg BW). The possible mechanism of activity of PF leaves extract may be stimulating the insulin secretion and regeneration of the β-cells of the pancreas or increasing cellularity of the islet tissue and regeneration of the granules in the β-cells. The entire animals used in the studies were kept in individual polyethylene cages and maintained standard condition (12 h dark and 12 h light circle; 25 ± 5°C; 40-60% humidity). [26–28]. Paederia Foetida Linn. Hu FB, Stampfer MJ, Haffner SM, Solomon CG, Willett WC, Manson JE: Elevated risk of cardiovascular disease prior to clinical diagnosis of type 2 diabetes. Histopathology studies of the experimental rat were measured after 28 days continuous administration of different doses of PF and glibenclamide. Three different doses of PF leaves extract (100, 250 and 500 mg/kg) to STZ induced diabetic rats caused significantly (P < 0.001) decline the blood glucose level which was showing dose and duration of treatment. For each group, 6 rats were examined and 50 pictures were taken. STZ induced diabetic rat increased the blood glucose level and decrease the insulin level due to STZ induced abnormalities in the β cell function [47]. The effects of the methanol extract (MLE) of Paederia foetida freshly isolated liver hepatocytes of rats intoxicated with Carbon tetrachloride were depicted in Table 1. Oral administration of the different doses of the PF (100, 250 and 500 mg/kg) showed significantly (P < 0.001) lower AUCglucose values as compared to the glucose control group rats (Table 1). The slit dimension was kept at 4.0 mm × 0.45 mm and 20 mm/s scanning speed was employed. The level of cholesterol, triglyceride, LDL (low density cholesterol) and VLDL (very low density cholesterol) were increased and the level of HDL (high density cholesterol) was decreased in the STZ induced diabetic rat as compared to the normal control group rats. Danish Ahmed. leaves extract effect on pancreas histopathology in normal and STZ induced diabetic rats. The antioxidant activity of extract was due to inhibition of lipid peroxidation and improved level of SOD, GPx and CAT. Groups C and D received EEPF 500 mg/kg body weight (BW) and 5-aminosalisylic acid … 1979, 132 (4): 289-297. Add. Our result lie in agreement with this well known fact that treatment with PF for 28 days decreased the excess level of the glycated hemoglobin (Table 2), which indicates the improved echelon of glycemic control. PF dose 500 mg/kg shown the maximum reduction of the high blood glucose level when compared to the two other different grades of doses of PF. Department of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom Institute of Agriculture, Technology & Sciences (SHIATS)-Deemed University, Allahabad, Uttar Pradesh, 211007, India, Sidharatha Institute of Pharmacy, Dehradun, Uttrakhand, India, Health Information Technology Department, Jeddah Community College, King Abdulaziz University, Jeddah, 21589, Saudi Arabia, Department of Pharmacology, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia, Department of Pathology, MLN Medical College Allahabad, Allahabad, Uttar Pradesh, India, Department of Biological Sciences, Sam Higginbottom Institute of Agriculture, Technology & Sciences (SHIATS)-Deemed University, Allahabad, Uttar Pradesh, India, Department of Phytochemisty & Pharmacognosym, Faculty of Pharmacy, Jamia Hamdard, New Delhi, 110062, India, You can also search for this author in Conclusions: These observations established the traditional claim and thus Paederia foetida could be a potent gastroprotective agent for use in future. According to the WHO (World Health Organization) an estimated 300 million during World, 57.2 million adults in India affected in the year 2025 [3, 4]. STZ induced diabetic rats has been shown to have increased level of fructose-1-6-biphosphate. Prasarni LEAF POWDER. Orhan N, Aslan M, Demirci B, Ergun F: A bioactivity guided study on the antidiabetic activity of Juniperus oxycedrus subsp. Based upon the result it can be hypothesized that PF declined the level of the blood glucose and improved the pancreas insulin level [45]. alcohol at Other graded dose of PF 250 mg/kg showed the improved effect on the liver histopathology on the accumulation of the fat droplet as compared to the PF dose 100 mg/kg and diabetic control group rat. The samples were obtained from the same liver anatomical regions. During oxidative stress increased production of Lipid peroxidation impairs the membrane function by decreasing membrane fluidity and changing the activity of membrane-bound enzyme and receptors [53]. This study is the first report on the determination and quantification of scopoletin using quantitative nuclear magnetic resonance (qNMR) in the different extracts of Paederia foetida twigs. The samples were obtained from the same liver anatomical regions. (E) PF III (500 mg/kg): PF tested drug display normal size of islet (Red arrow) (F) Glibenclamide (10 mg/kg): Glibenclamide treated group rat histopathology display normal islet (Red arrow) similar to the normal control group. All the animals were tested for absolute and relative organ weight and gross pathological lesions. The PF dose had shown the best result when compared to the synthetic drug glibenclamide (Table 2). The other enzymatic antioxidant CAT is a hemoprotein, which cause reduction of the hydrogen peroxide and known to be involved in the detoxification of hydrogen peroxide concentration [55]. Abdominal Pain and flatus ; Oberley LW, Oberley TD: Role of antioxidant enzymes in cell immortalization and transformation. STZ induced diabetic group rat disturbed the lipid profile and showed direct effect on the heart. Oxygen free radical plays an important role in the production of the oxidative stress. Google Scholar. Administration of PF dose 500 mg/kg in the normal rat showed little or no changes in the level of hexokinase. Scavenging of reactive oxygen species (ROS) may be attributed to the high amount of phenolic compounds present in the extract. Further, increase in the blood glucose level in diabetic patient causes alteration in utilization of glucose in the vital organs viz. About 30 species of Paederia are recognized worldwide, with a disjunct distribution covering continental tropical Asia (the putative centre of origin), Malesia, Madagascar and East Africa, as well as tropical America (Nie et al., 2013). PubMed Central  Privacy 2006, 112: 1-6. In the diabetes condition hypertriglyceridemia and hypercholestermia are the common factors involved in the development of the atherosclerosis and coronary heart disease. Antidiarrhoeal activity of 90% ethanol extract of Paederia foetida was investigated in this study using castor oil and magnesium sulphate-induced diarrhoea models in mice. Fructose-1-6-biphosphateis the other vital enzyme convert glucose into energy and plays an important role in the glycolysis [60, 61]. Oral administration of different doses of PF was significantly decreased the level of glycated haemoglobin (A1c) in STZ-induced treated diabetic rats (Table 2). 10.2337/diacare.21.9.1414. Group I: rats was treated with vehicle only. There collected blood sample was used to determine the antihyperlipidemic, hypoglycemic and antioxidant parameters. Google Scholar. PubMed  (E) PF III (500 mg/kg): Did not display any changes of the heart histopathology (F) Glibenclamide (10 mg/kg): Histopathology similar to the normal group. Paederia foetida Linn. Ind J Pharmco. BMC Complement Altern Med. (F) Glibenclamide (10 mg/kg): Glibenclamide treated group rat display the normal histopathology. Streptozotocin (2-deoxy-2-(N-methyl-N-nitrsourea) -1-D-glucopyranose) is a nitrosourea compound obtained from the soil microbe Streptomyces achromogenes[42], which is the potent alkylating agent that exerts its effect on the pancreatic β-cells via glucose transporter-GLUT2, causing induction of methylation of DNA and results in damage of DNA. Apocynum foetidum Burm.f.. Gentiana scandens Lour.. Hondbesseion tomentosum (Blume) Kuntze. 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